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Image Search Results
Journal: Clinical Cancer Research
Article Title: IFNα Augments Clinical Efficacy of Regulatory T-cell Depletion with Denileukin Diftitox in Ovarian Cancer
doi: 10.1158/1078-0432.ccr-20-4594
Figure Lengend Snippet: Figure 5. DCs mediate IFNa effects on Treg suppression through IL6. A, Flow cytometric analysis of suppression of T-cell proliferation by Tregs from the PEC of ID8- challenged IFNR/ mice adoptively transferred with WT or IFNR/ DCs and then treated with DD plus IFNa (samples pooled for each experiment, all groups n ¼ 3). B, Luminex analysis of IL6 and IL10 in supernatant with Tregs, DCs and/or IFNa (one of three experiments with similar results). Suppression of T-cell proliferation by Tregs in the presence of DCs, IFNa, and/or anti-IL6 (n ¼ 3 for all groups; C) or in the presence of DCs, IFNa, and/or recombinant IL6 (n ¼ 3 for all groups; D). E, Suppression of T-cell proliferation by Tregs in the presence of DCs, recombinant IL6, anti-CD80 and/or anti-CD86 (n ¼ 3 for all groups). F, Treg function in mLNs of mice challenged with ID8, treated with DDþIFNa PBS or anti-IL6 in vivo (n ¼ 5 for both groups).
Article Snippet:
Techniques: Luminex, Recombinant, In Vivo
Journal: Clinical Cancer Research
Article Title: IFNα Augments Clinical Efficacy of Regulatory T-cell Depletion with Denileukin Diftitox in Ovarian Cancer
doi: 10.1158/1078-0432.ccr-20-4594
Figure Lengend Snippet: Figure 7. Pegylated IFNa has activity in the clinical response to failed DD in patients with ovarian cancer. A, CA-125 serum levels in patient 103. IFNa given as weekly, subcutaneous pegylated IFNa2a. B, CA-125 serum levels in patient 105. C, Serum IL6 in patient 103 after treatment with DD alone and after addition of pegylated IFNa2a. Mean of triplicate replicates. D, Flow cytometric analysis of Treg prevalence (CD4þCD25hiCD127lo cells among all blood CD3þCD4þ
Article Snippet:
Techniques: Activity Assay